Efficient Method for the Rapid Purification of Nosema ceranae Spores
نویسندگان
چکیده
منابع مشابه
Efficient Method for the Rapid Purification of Nosema ceranae Spores
Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we con...
متن کاملA serological method for detection of Nosema ceranae.
AIMS We developed a new method for detection of the intracellular parasite, Nosema ceranae, one of the most economically devastating pathogens of the honeybee. METHODS AND RESULTS The SWP-32 antibody was used for the development of an enzyme-linked immunosorbent assay (ELISA). We also compared the efficiency of this ELISA to microscopy and quantitative real-time (qRT) PCR, the methods current...
متن کاملEvaluation of large-scale dissemination of Nosema ceranae spores by European bee-eaters Merops apiaster.
Identification of transmission routes and of factors affecting the spatial positions of pathogens, hosts and vectors is basic to an adequate disease management. Nosema ceranae is a Microsporidian recently described as a parasite of Apis mellifera honeybees and is currently considered the aetiological agent of an emergent illness named nosemosis type C. In this article we evaluate the role of a ...
متن کاملDoes Nosema ceranae Wipe Out Nosema apis in Turkey?
BACKGROUND The aim of this study was to determine the prevalence of the Nosema ceranae and Nosema apis among apiaries using both spore counts and multiplex PCR and the replacement of N. apis by N. ceranae in some regions of Turkey. METHODS A hundred honey bee samples were collected from 99 apiaries in 11 different locations in 2011-2012 in Turkey. Nosema infection degree from collected sample...
متن کاملFirst Detection of Nosema Ceranae in Jordan
Nosema diseases is known to have two infecting Apis mellifera, Nosema apis and Nosema ceranae. Historically N. apis was known to be well established pathogen of A. mellifera. Whoever N. ceranae was frequently reported to infect the honeybees during the last decade. Both N. apis and N. ceranae are now thought to be widespread across the world. Honey bees are also found to be co-infected with bot...
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ژورنال
عنوان ژورنال: Mycobiology
سال: 2017
ISSN: 1229-8093,2092-9323
DOI: 10.5941/myco.2017.45.3.204